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nk cell isolation kit  (Miltenyi Biotec)


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    Miltenyi Biotec nk cell isolation kit
    Nk Cell Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 2265 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+nk+cell+isolation+kit/NK+Cell+Isolation+Kit%2C+human/pm42526444-580-20-25
    Average 97 stars, based on 2265 article reviews
    nk cell isolation kit - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Isolation:

    Article Title: ADAM17 blockade restores natural killer cell antibody-dependent cytotoxicity against human immunodeficiency virus-1.
    Article Snippet: .. In parallel, NK cells were isolated from PBMCs following overnight incubation using the human NK cell isolation kit (Miltenyi Biotec). .. To prepare target cells, BaL-uninfected or BaL-infected cells were stained with 50 nM CFSE (ThermoFisher Scientific) for 20 minutes, and cells were incubated with anti-human CD4 antibody (Nonhuman Primate Reagent Resource; clone CD4R1) 48 or anti–HIV-1 env antibody A32 (kindly donated by the laboratory of Dr. Guido Ferrari at Duke University) 26 at room temperature for 15 minutes.

    Article Title: Nur77 agonism invigorates Natural Killer cell immunity against hepatocellular carcinoma.
    Article Snippet: Purified PBMCs were re-suspended in MACS buffer [1x PBS (Gibco) with 2mM EDTA (1st Base) and 0.5% FBS (Thermo Fisher Scientific)]. .. Isolation and expansion of human Natural Killer cells from PBMCs NK cell isolation was done with the human NK cell isolation kit (Miltenyi Biotec) according to manufacturer’s protocol. .. Red blood cell (RBC) lysis was performed using ACK lysis buffer (Thermo Fisher Scientific).

    Article Title: Droplet microfluidic profiling of NK cell cytotoxicity with machine learning-enabled target-cell death analysis.
    Article Snippet: .. Peripheral blood NK cells were isolated from fresh blood of three female donors using a commercial human NK Cell Isolation Kit (negative selection; Miltenyi Biotec, Germany; Cat. 130-092-657) according to the manufacturer's protocol. .. NK-cell purity was confirmed by flow cytometry using the following antibodies (BD Biosciences): anti-human CD45 (clone HI30, purified; Cat. 555480), CD56 (clone MY31, PE; Cat. 556647), and CD3 (clone OKT3, PE; Cat. 566684).

    Article Title: Efficient NK cell transduction with VSV-G-pseudotyped lentiviral vectors
    Article Snippet: .. NK cells were isolated from random healthy buffy coats using the Human NK Cell Isolation Kit (Miltenyi Biotec, Germany) and the manufacturer’s instructions. ..

    Article Title: Optimization of head and neck cancer peripheral blood mononuclear cell processing for robust downstream functional immune analysis
    Article Snippet: .. NK cells were isolated from PBMCs per manufacturer’s instructions using the human NK cell isolation kit (Miltenyi, cat130-092-657). ..

    Article Title: Cytokine preactivation and ADCC: a potent strategy for enhancing human NK cell effector functions against 3D tumor models
    Article Snippet: Peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats via Ficoll ® Paque Plus (GE Healthcare) density gradient centrifugation, as previously described ( ). .. Freshly isolated PBMCs were either cryopreserved for later use ( ) or immediately processed for NK cell isolation using the Human NK Cell Isolation Kit (Miltenyi Biotec). ..

    Incubation:

    Article Title: ADAM17 blockade restores natural killer cell antibody-dependent cytotoxicity against human immunodeficiency virus-1.
    Article Snippet: .. In parallel, NK cells were isolated from PBMCs following overnight incubation using the human NK cell isolation kit (Miltenyi Biotec). .. To prepare target cells, BaL-uninfected or BaL-infected cells were stained with 50 nM CFSE (ThermoFisher Scientific) for 20 minutes, and cells were incubated with anti-human CD4 antibody (Nonhuman Primate Reagent Resource; clone CD4R1) 48 or anti–HIV-1 env antibody A32 (kindly donated by the laboratory of Dr. Guido Ferrari at Duke University) 26 at room temperature for 15 minutes.

    Article Title: Cord blood natural killer cell-derived extracellular vesicles inhibit Zika virus infectivity through ITGB2/perforin-mediated envelope disruption in vitro and in vivo
    Article Snippet: .. Under a sequential MACS strategy for untouched NK cell isolation, PBMCs are first subjected to negative selection using the human NK Cell Isolation Kit (Miltenyi Biotec, cat130-092-657), which depletes CD3 + T cells and other non-NK lineages; the resulting enriched flow-through is then immediately used for positive selection via incubation with human CD56 Microbeads (Miltenyi Biotec, cat#130-050-401), followed by a final magnetic separation to obtain a highly pure population of CD3 − CD56 + NK cells. .. Freshly isolated NK cells were seeded at an initial density of 0.5–1.0 × 10 6 cells/mL in serum-free NK cell-specific medium (Lonza NKMACOTM Medium, cat#BE001NC), supplemented with 500IU/mL recombinant human IL-2 (PeproTech, cat#200–02) and 10 ng/mL recombinant human IL-15 (PeproTech, cat#200–15).

    Cell Isolation:

    Article Title: ADAM17 blockade restores natural killer cell antibody-dependent cytotoxicity against human immunodeficiency virus-1.
    Article Snippet: .. In parallel, NK cells were isolated from PBMCs following overnight incubation using the human NK cell isolation kit (Miltenyi Biotec). .. To prepare target cells, BaL-uninfected or BaL-infected cells were stained with 50 nM CFSE (ThermoFisher Scientific) for 20 minutes, and cells were incubated with anti-human CD4 antibody (Nonhuman Primate Reagent Resource; clone CD4R1) 48 or anti–HIV-1 env antibody A32 (kindly donated by the laboratory of Dr. Guido Ferrari at Duke University) 26 at room temperature for 15 minutes.

    Article Title: Nur77 agonism invigorates Natural Killer cell immunity against hepatocellular carcinoma.
    Article Snippet: Purified PBMCs were re-suspended in MACS buffer [1x PBS (Gibco) with 2mM EDTA (1st Base) and 0.5% FBS (Thermo Fisher Scientific)]. .. Isolation and expansion of human Natural Killer cells from PBMCs NK cell isolation was done with the human NK cell isolation kit (Miltenyi Biotec) according to manufacturer’s protocol. .. Red blood cell (RBC) lysis was performed using ACK lysis buffer (Thermo Fisher Scientific).

    Article Title: Cord blood natural killer cell-derived extracellular vesicles inhibit Zika virus infectivity through ITGB2/perforin-mediated envelope disruption in vitro and in vivo
    Article Snippet: .. Under a sequential MACS strategy for untouched NK cell isolation, PBMCs are first subjected to negative selection using the human NK Cell Isolation Kit (Miltenyi Biotec, cat130-092-657), which depletes CD3 + T cells and other non-NK lineages; the resulting enriched flow-through is then immediately used for positive selection via incubation with human CD56 Microbeads (Miltenyi Biotec, cat#130-050-401), followed by a final magnetic separation to obtain a highly pure population of CD3 − CD56 + NK cells. .. Freshly isolated NK cells were seeded at an initial density of 0.5–1.0 × 10 6 cells/mL in serum-free NK cell-specific medium (Lonza NKMACOTM Medium, cat#BE001NC), supplemented with 500IU/mL recombinant human IL-2 (PeproTech, cat#200–02) and 10 ng/mL recombinant human IL-15 (PeproTech, cat#200–15).

    Article Title: Droplet microfluidic profiling of NK cell cytotoxicity with machine learning-enabled target-cell death analysis.
    Article Snippet: .. Peripheral blood NK cells were isolated from fresh blood of three female donors using a commercial human NK Cell Isolation Kit (negative selection; Miltenyi Biotec, Germany; Cat. 130-092-657) according to the manufacturer's protocol. .. NK-cell purity was confirmed by flow cytometry using the following antibodies (BD Biosciences): anti-human CD45 (clone HI30, purified; Cat. 555480), CD56 (clone MY31, PE; Cat. 556647), and CD3 (clone OKT3, PE; Cat. 566684).

    Article Title: Efficient NK cell transduction with VSV-G-pseudotyped lentiviral vectors
    Article Snippet: .. NK cells were isolated from random healthy buffy coats using the Human NK Cell Isolation Kit (Miltenyi Biotec, Germany) and the manufacturer’s instructions. ..

    Article Title: HLA-B51 induces IFN- γ production in human natural killer cells
    Article Snippet: After centrifugation at 1,800 rpm for 15 min at 27°C, the mononuclear cell layer was collected, suspended in PBS, and centrifuged again at 1,600 rpm for 5 min. Pellets were washed with PBS again by centrifugation at 1,400 rpm for 5 min and resuspended with DMEM (Nacalai Tesque). .. NK cells were separated from peripheral blood mononuclear cells (PBMCs) using the autoMACS system with human NK Cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s procedures. ..

    Article Title: Optimization of head and neck cancer peripheral blood mononuclear cell processing for robust downstream functional immune analysis
    Article Snippet: .. NK cells were isolated from PBMCs per manufacturer’s instructions using the human NK cell isolation kit (Miltenyi, cat130-092-657). ..

    Article Title: Cytokine preactivation and ADCC: a potent strategy for enhancing human NK cell effector functions against 3D tumor models
    Article Snippet: Peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats via Ficoll ® Paque Plus (GE Healthcare) density gradient centrifugation, as previously described ( ). .. Freshly isolated PBMCs were either cryopreserved for later use ( ) or immediately processed for NK cell isolation using the Human NK Cell Isolation Kit (Miltenyi Biotec). ..

    Magnetic Cell Separation:

    Article Title: Cord blood natural killer cell-derived extracellular vesicles inhibit Zika virus infectivity through ITGB2/perforin-mediated envelope disruption in vitro and in vivo
    Article Snippet: .. Under a sequential MACS strategy for untouched NK cell isolation, PBMCs are first subjected to negative selection using the human NK Cell Isolation Kit (Miltenyi Biotec, cat130-092-657), which depletes CD3 + T cells and other non-NK lineages; the resulting enriched flow-through is then immediately used for positive selection via incubation with human CD56 Microbeads (Miltenyi Biotec, cat#130-050-401), followed by a final magnetic separation to obtain a highly pure population of CD3 − CD56 + NK cells. .. Freshly isolated NK cells were seeded at an initial density of 0.5–1.0 × 10 6 cells/mL in serum-free NK cell-specific medium (Lonza NKMACOTM Medium, cat#BE001NC), supplemented with 500IU/mL recombinant human IL-2 (PeproTech, cat#200–02) and 10 ng/mL recombinant human IL-15 (PeproTech, cat#200–15).

    Selection:

    Article Title: Cord blood natural killer cell-derived extracellular vesicles inhibit Zika virus infectivity through ITGB2/perforin-mediated envelope disruption in vitro and in vivo
    Article Snippet: .. Under a sequential MACS strategy for untouched NK cell isolation, PBMCs are first subjected to negative selection using the human NK Cell Isolation Kit (Miltenyi Biotec, cat130-092-657), which depletes CD3 + T cells and other non-NK lineages; the resulting enriched flow-through is then immediately used for positive selection via incubation with human CD56 Microbeads (Miltenyi Biotec, cat#130-050-401), followed by a final magnetic separation to obtain a highly pure population of CD3 − CD56 + NK cells. .. Freshly isolated NK cells were seeded at an initial density of 0.5–1.0 × 10 6 cells/mL in serum-free NK cell-specific medium (Lonza NKMACOTM Medium, cat#BE001NC), supplemented with 500IU/mL recombinant human IL-2 (PeproTech, cat#200–02) and 10 ng/mL recombinant human IL-15 (PeproTech, cat#200–15).

    Article Title: Droplet microfluidic profiling of NK cell cytotoxicity with machine learning-enabled target-cell death analysis.
    Article Snippet: .. Peripheral blood NK cells were isolated from fresh blood of three female donors using a commercial human NK Cell Isolation Kit (negative selection; Miltenyi Biotec, Germany; Cat. 130-092-657) according to the manufacturer's protocol. .. NK-cell purity was confirmed by flow cytometry using the following antibodies (BD Biosciences): anti-human CD45 (clone HI30, purified; Cat. 555480), CD56 (clone MY31, PE; Cat. 556647), and CD3 (clone OKT3, PE; Cat. 566684).



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